向凤宁
E-Mail：xfn0990@sdu.edu.cn
个人简介
向凤宁，9159金沙游戏场特聘教授，二级教授，博士生导师。教育部新世纪优秀人才、山东省杰出青年及青岛市拔尖人才。中国植物生理学会理事、中国大豆专业委员会理事、中国细胞生物学学会植物器官发生分委会委员、中国植物生理与分子生物学学会女科学家分会委员。长期从事大豆产量及耐逆性状形成的分子机理及种质创新研究，承担“十四五”国家重点研发计划、国家重大科学研究计划子课题（973）、国家转基因重大专项重点课题、NSFC-山东联合基金等课题20余项，近年来，在Nature Communications、Plant Cell、Plant physiology、Plant Journal等期刊上发表论文100余篇（SCI论文60余篇）。授权国家发明专利26项。2006年获山东省自然科学奖“一等奖”、2009年获教育部技术发明“二等奖”。培育出高产耐逆大豆新品种——“山大5号”，并实现了成果转化。
教育背景
2006-2007 韩国首尔大学 植物分子生物学博士后
2000-2003 9159金沙游戏场 发育生物学博士
1986-1989 9159金沙游戏场 植物学硕士
1982-1986 青海师范大学 生物学学士
工作经历
2003-至今 9159金沙游戏场 教授（博导）
1998-2003 9159金沙游戏场 副教授
1997-1998 9159金沙游戏场 讲师
科研方向
大豆是我国战略性经济作物，进口依存度达到85%以上，大豆产业安全面临危机，因此，发掘大豆株型和耐盐碱关键基因，解析其作用机制，培育高产/耐盐碱大豆新品种刻不容缓。课题组长期围绕大豆产量及耐逆性状形成的分子机理及种质创新开展研究，创建了用于大规模基因发掘的大豆突变体库，基于图位克隆及BSA seq技术发掘出多个调控株型及耐盐碱关键基因；构建了基于全基因组关联分析（GWAS）大规模发掘功能基因的平台：完成了591种大豆/野大豆全基因组重测序，鉴定了1000多个大豆及野大豆种质田间产量性状及耐盐表型组数据，基于GWAS筛选出一批调控株型及耐盐碱候选基因；创建了大豆功能基因作用机制研究平台：系列报道多个大豆株型及耐逆基因功能及其调控机制（Nature Communications、Plant Cell、Plant physiology等）；建立了高效的大豆基因转化和基因编辑技术系统，为分子育种奠定基础。在此基础之上，研究方向主要包括：
1、大豆耐盐碱关键基因发掘及其作用机制
耐盐碱是实现大豆种植面积扩大的有效途径，利用大豆自然群体和突变体，分离调控耐盐碱主效基因、阐明其分子机制和调控网络，鉴定耐盐碱基因的优异等位变异，为大豆耐盐分子设计育种提供分子标记和编辑靶点，创制高产、耐盐碱大豆新种质。
2、大豆株型性状形成的分子机理
密植是实现大豆高产的关键途径，聚焦调控耐密植（分枝、株高、抗倒伏）主效基因及其优异等位基因的分离，阐明其分子调控网络，创制高产、耐密植大豆新种质。
（招收以上研究方向的硕士、博士研究生和博士后研究人员）
主持课题
1.2023-2025，耐盐碱高产大豆新品种设计与培育，农业生物育种重大项目（主持）
2.2021-2024，大豆高产性状形成的分子调控机理，国家重点研发计划（主持）
3.2020-2023，大豆耐盐新基因作用机制及利用研究，NSFC-山东联合基金（主持）
4.2016-2020，环境胁迫应答调控元件的分离鉴定，国家转基因重大专项（主持）
5.2018-2020，大豆抗旱／耐盐重要功能基因发掘及种质创新，国家转基因重大专项（主持）
6.2013-2017，微环境调控植物生长点干细胞中心维持与重建的分子网络，国家重大科学研究计划（“973”）项目（主持）
7.2013-2017，大豆高产抗逆功能基因组研究，国家“863”项目（主持）
8.2018-2021，拟南芥miR159调控离体干细胞中心重建的分子机制研究，国家自然科学基金面上项目（主持）
9.2015-2018，大豆膜结合转录因子GmNTLx在大豆耐盐中的作用机制研究， 国家自然科学基金面上项目（主持）
10.2013-2016，拟南芥生长素响应因子ARF10 调控离体苗再生的分子机制，国家自然科学基金面上项目（主持）
11.2021-2024，大豆育种关键技术研发与新品种选育，山东省农业良种工程项目（主持）
12.2023-2026，耐盐碱大豆突破性新品种培育，山东省农业良种工程项目（主持）
代表性研究成果
*Corresponding author（通讯作者）
1.Jiaqi Sun1, Shiyu Huang1, Qing Lu1, Shuo Li1, Shizhen Zhao1, Xiaojian Zheng1, Qian Zhou1, Wenxiao Zhang1, Jie Li1, Lili Wang1, Ke Zhang1, Wenyu Zheng1, Xianzhong Feng2*, Baohui Liu3*, Fanjiang Kong3* and Fengning Xiang1*. UV-B irradiation-activated E3 ligase GmILPA1 modulates gibberellin catabolism to increase plant height in soybean. Nature Communications. 2023. 14:6262. 1-17 https://doi.org/10.1038/s41467-023-41824-3.
2.Wenxiao Zhang1, Wenjiao Zhi1, Hong Qiao1, Jingjing Huang2,3, Shuo Li1, Qing Lu 1, Nan Wang1, Qiang Li1, Qian Zhou1, Jiaqi, Sun1, Yuting Bai1, Xiaojian Zheng1, Mingyi Bai1, Frank Van Breusegem2,3, Fengning Xiang1*. H2O2-dependent oxidation of the transcription factor GmNTL1 promotes salt tolerance in soybean. The Plant Cell. 2023: 1-24.
3.Zhenhua Liu#, Xuehuan Dai#, Juan Li#&, Na Liu, Xiangzheng Liu, Shuo Li, and Fengning Xiang*, The Type-B Cytokinin Response Regulator ARR1 Inhibits Shoot Regeneration in an ARR12-Dependent Manner in Arabidopsis. The Plant Cell. 2020. 32: 2271–2291 (Cover Story)
4.Shuo Li†,1, Nan Wang†,1, Dandan Ji†,1,#, Wenxiao Zhang†,1, Ying Wang1, Yanchong Yu1, Shizhen Zhao1, Menghua Lyu1, Juanjuan You1, Yangyang Zhang1, Luli Wang1, Xiaofang Wang1, Zhenhua Liu1, Jianhua Tong2, Langtao Xiao2, Ming-yi Bai1 and Fengning Xiang*,1. A GmSIN1/GmNCED3s/GmRbohBs feed-forward loop acts as a signal amplifier that regulates root growth in Soybean exposed to salt stress. The Plant Cell. 2019. 31(9):2107-2130.
5.Shuo Li†, Nan Wang†,Dandan Ji†, Zheyong Xue, Yanchong Yu, Yupei Jiang, Jinglin Liu, Zhenhua Liu and Fengning Xiang1*. Evolutionary and functional analysis of membrane-bound NAC transcription factor genes in soybean. Plant physiology. 2016. (172): 1805-1820.
6.XuehuanDai1,2, JingWang1, Lili Wang1, Zhenhua Liu1,3, Qiang Li1, Yunfei Cai1,4, Shuo Li1 and Fengning Xiang1,*. HY5 inhibits in vitro shoot stem cell niches initiation via directly repressing pluripotency and cytokinin pathways. The Plant Journal, 2022. 110:781–801.
7.Yanchong Yu1,*, Yasnan Qi1, Jinpeng Xu1, Xuehuan Dai2, Jiacai Chen1, Chun-Hai Dong1,* and Fengning Xiang2,*. Arabidopsis WRKY71 regulates ethylene-mediated leaf senescence by directly activating EIN2, ORE1 and ACS2 genes. The Plant Journal. 2021. 107: 1819–1836.
8.Xuehuan Dai1†, Jing Wang1†, Yuguang Song1,2†, Zhenhua Liu1,3, Tao Xue1,4, Meng Qiao1, Yanchong Yu1,5, Wei Xin1,6 and Fengning Xiang1*. Cytosine methylation of the FWA promoter promotes direct in vitro shoot regeneration in Arabidopsis thaliana. JIPB (Journal of Integrative Plant Biology). 2021. 63(8):1491–1504.
9.Yanchong Yu1,†, Zhenhua Liu1,†, Long Wang1,†, Sang-Gyu Kim2, Pil J. Seo2,, Meng Qiao1, Nan Wang1, Shuo Li1,Xiaofeng Cao3, Chung-Mo Park2 and Fengning Xiang1,*. WRKY71 accelerates flowering via the direct activation of FLOWERING LOCUS T and LEAFY in Arabidopsis thaliana. The Plant Journal. 2016. 85: 96–106.
10. Zhenhua Liu1†, Juan Li1†, Qiang Li1, Long Wang1, Qing Lu1, Yanchong Yu1, Shuo Li1, Ming-yi Bai1, Yuxin Hu2 and Fengning Xiang1*. Repression of callus initiation by the miRNA-directed interaction of auxin-cytokinin in Arabidopsis thaliana. The Plant Journal. 2016. 87: 391–40.
11. Meng Qiao, Zhongjuan Zhao, Yuguang Song, Zhenhua Liu, Lingxue Cao, Yanchong Yu, Shuo Li and Fengning Xiang*. Proper regeneration from in vitro cultured Arabidopsis thaliana requires the miRNA directed action of an auxin response factor. The Plant Journal. 2012. 71, 14–22.
12. Pil Joon Seo,1 Fengning Xiang1(co-first author), Meng Qiao, Ju-Young Park, Sang-Gyu Kim, Yong-Hwan Lee, and Chung-Mo Park. The MYB96 Transcription Factor Mediates Abscisic Acid Signaling during Drought Stress Response in Arabidopsis. Plant Physiology. 2009. 151(1):275-89.
13. Xuehuan Dai1, Zhenhua Liu1, Meng Qiao2, Juan Li1, Shuo Li1 and Fengning Xiang1,*. ARR12 promotes de novo shoot regeneration in Arabidopsis thaliana via activation of WUSCHEL expression. JIPB (Journal of Integrative Plant Biology). 2017. 59(10): 747–758.
14. Zhao Cuizhu, Qiao Meng, Yu Yanchong, Xia Guangmin, Xiang Fengning*. The effect of the heterologous expression of Phragmites australis γ-glutamylcysteine synthetase on the Cd2+ accumulation of Agrostis palustris. Plant Cell & Environment. 2010. 33: 877–887.
15. Yanchong Yu1,2, Long Wang1, Jiacai Chen2, Zhenhua Liu1, Chung-Mo Park3 and Fengning Xiang1,*. WRKY71 Acts Antagonistically against Salt-delayed Flowering in Arabidopsis thaliana. Plant & Cell Physiology. 2018, 59(2): 414–422.
16. Nan Wang1, Wenxiao Zhang1, Mengyin Qin1, Shuo Li1, Meng Qiao2, Zhenhua Liu1 and Fengning Xiang1,*. Drought tolerance conferred in soybean (Glycine max. L) by GmMYB84, a novel R2R3-MYB transcription factor. Plant & Cell Physiology. 2017. 58(10): 1764–1776.
17. Tao Xue, Xuehuan Dai, Ruipu Wang, Junzhu Wang, Zhenhua Liu* and Fengning Xiang*. ARGONAUTE10 Inhibits In Vitro Shoot Regeneration Via Repression of miR165/166 in Arabidopsis thaliana. Plant & Cell Physiology. 2017. 58(10):1789-1800.
18. Wenxiao Zhang1, Nan Wang1, Jingting Yang, Hui Guo, Zhenhua Liu, Xiaojian Zheng, Shuo Li*, Fengning Xiang*. The salt-induced transcription factor GmMYB84 confers salinity tolerance in soybean. Plant Science. 2020. 291:110326.
19. Xuehuan Dai1, Na Liu1, Lijuan Wang, Juan Li2, Xiaojian Zheng, Fengning Xiang*, Zhenhua Liu*. MYB94 and MYB96 additively inhibit callus formation via directly repressing LBD29 expression in Arabidopsis thaliana. Plant Science. 2020. 293:110323.
20. Long Wanga, Zhenhua Liua, Meng Qiaob, Fengning Xianga,*. miR393 inhibits in vitro shoot regeneration in Arabidopsis thaliana via repressing TIR1. Plant Science. 2018. 266:1-8.
21. Tao Xue, Zhenhua Liu, Xuehuan Dai, Fengning Xiang*. Primary root growth in Arabidopsis thaliana is inhibited by the miR159 mediated repression of MYB33, MYB65 and MYB101. Plant Science. 2017. 262: 182–189.
22. Dexin Wanga,b,c,1, Yanchong Yua,1, Zhenhua Liua, Shuo Lia, Zeli Wangb,* and Fengning Xianga,*. Membrane-bound NAC transcription factors in maize and their Contribution to the oxidative stress response. Plant Science. 2016.250:30–39.
23.Xuehuan Dai, Qing Lu, Jing Wang, Lili Wang, Fengning Xiang, Zhenhua Liu*. MiR160 and its target genes ARF10, ARF16 and ARF17 modulate hypocotyl elongation in a light, BRZ, or PAC-dependent manner in Arabidopsis: miR160 promotes hypocotyl elongation. Plant Science. 2021.303:110686.
24. Yanling Liu, Zhongjuan Zhao, Zhongjuan Zhao, Zheyong Xue, Long Wang, Yunfei Cai, Peng Wang, Tiandi Wei, Jing Gong and Fengning Xiang,*. An Intron-less β-amyrin Synthase Gene is More Important on Oleanolic Acid Accumulation than its Paralog in Gentiana straminea. Scientific Report. 2016. 6:33364: 1-13.
25. Junfeng Wang, Cuizhu Zhao, Chang Liu, Guangmin Xia and Fengning Xiang*. Introgression of Swertia mussotii gene into Bupleurum scorzonerifolium via somatic hybridization. BMC plant biology. 2011. 11:71-81.
26. Cuizhu Zhao, Jin Xu, Qiang Li, Shuo Li, Peng Wang and Fengning Xiang*. Cloning and characterization of a Phragmites australis phytochelatin synthase (PaPCS) and achieving Cd tolerance in tall fescue. PLoS ONE. 2014.9(8):1-12.
27. Yuguang Song, Dandan Liu, Shuo Li and Fengning Xiang*. The dynamic changes of DNA methylation and histone modifications of salt responsive transcription factor genes in soybean. PLoS ONE. 2012.7(7):1-8
28. Fengning Xiang, Junfeng Wang, Chunhui Xu, Guangmin Xia*. The chromosome content and genotype of two wheat cell lines and of their somatic fusion product with oat. Planta. 2010. 231:1201–1210
29.Meng Qiao and Fengning Xiang*. A set of Arabidopsis thaliana miRNAs involve shoot regeneration in vitro. Plant Signaling & Behavior. 2013. e23479-1-3
30.Gaobin Pu, Bingqian Zhou & Fengning Xiang*. Isolation and functional characterization of a Lonicera japonica hydroxycinnamoyl transferase involved in chlorogenic acid synthesis. Biologia. 2017. 72(6): 608—618
31.Yanchong Yu, Nan Wang, Ruibo Hu and Fengning Xiang*. Genome-wide identification of soybean WRKY transcription factors in response to salt stress. Springer Plus. 2016. DOI 10.1186/s40064-016-2647-x
32.Yunfei Cai, Guanghua Liu, Yang Yu and Fengning Xiang*, Genotyping and metabolite characterization of somatic hybrids between Arabidopsis thaliana and Swertia mussotii. In vitro plant. 2015. 51:360–368
33.Zhenhua Liu, Wei Xin, Dandan Ji, Long Wang, Juan Li and Fengning Xiang*. GUS activity for miR165a/166b, REV and WUS/CLV3 in in vitro direct Arabidopsis thaliana shoot regeneration. Protoplasma. 2013. 250:1213–1218
34.Gaobin Pua, Peng Wang, Bingqian Zhou, Zhenhua Liu, Fengning Xiang*. Cloning and characterization of Lonicera japonica p-coumaroyl ester 3’-hydroxylase which is involved in the biosynthesis of chlorogenic acid. Biosci.Biotechnol.Biochem. 2013. 77（7）:1403-1409
35.Fangfang Zhang, Peng Wang, Guangwen Kang, Fengning Xiang*. Asymmetric protoplast fusions between Bupleurum scorzonerifolium Willd and Taxus chinensis var. mairei. Plant cell report. 2011. 30:1857–1864
36.Yanchong Yu, Zidong Li, Peng Wang, Fengning Xiang*. Genetic and biochemical characterization of somatic hybrids between Bupleurum scorzonerifolium and Gentianopsis paludosa. Protoplasma. 2012. 249:1029–1035
37.Li Jiang, Yunfei Cai, Guangmin Xia, Fengning Xiang*. Introgression of the heterologous nuclear DNAs and efficacious compositions from Swertia tetraptera into Bupleurum scorzonerifolium Willd. via somatic hybridization. Protoplasma. 2012. 249:737–745
38.Meng Qiao, Zhongjuan Zhao and Fengning Xiang*. Arabidopsis thaliana in vitro shoot regeneration is impaired by silencing of TIR1. Biologia Plantarum. 2012. 56 (3): 409-414
39.Wei Xin, Zhenhua Liu, Yuguang Song and Fengning Xiang*. Direct shoot regeneration from Arabidopsis thaliana shoot apical meristems. Biologia Plantarum. 2012. 56 (4): 601-606
40.Zhong-juan Zhao§, Yu-guang Song§, Yan-ling Liu§, Meng Qiao, Feng-ning Xiang*. The effect of elicitors on oleanolic acid accumulation and the expression of triterpenoid synthesis genes in Gentiana straminea. Biologia Plantarum. 2012.57 (1): 139-143
41.Junfeng Wang, Yanling Liu, Yunfei Cai, Guangmin Xia, Fengning Xiang*. Cloning and functional analysis of geraniol 10-hydroxylase, a cytochrome P450 from Swertia mussotii. Biosci.Biotechnol.Biochem. 2010. 74 (8):1583-1590
42.Yanling Liu, Yunfei Cai, Zhongjuan Zhao, Junfeng Wang, Jing Li, Wei Xin, Guangmin Xia, Fengning Xiang*. Cloning and functional analysis of aβ-amyrin Synthase Gene associated with oleanolic acid biosynthsis in Gentiana straminea. Biol. Pharm. Bull. 2009.32(5):818-824
43.Cai Yunfei, Yanling Liu, Zhenhua Liu, Feng Zhang, Xiang Fengning*, Xia Guangmin. High-frequency embryogenesis and regeneration of plants with high content of gentiopicroside from the Chinese medicinal plant Gentiana straminea Maxim. In vitro plant. 2009. 45:730–739
44.Lihua Zang, Yanling Liu, Jiaqing Liu, Qing Tian, Fengning Xiang*. An Improved Method for Testing Weak VOCs in Dry Plants with a Purge and Trap Concentrator Coupled to GC-MS. CHROMATOGRAPHIA. 2008. 68:351-356
45.Jing Wang1, Fengning Xiang1 (co-first author), Guangmin Xia. Agropyron elongatum chromatin localization on the wheat chromosomes in an introgressed somatic hybrid line. Planta. 2005. 221: 277-286
46.Fengning Xiang, Xia Guangmin, Chen Huimin. Hybrid plant regeneration in relation to the nuclear and cytoplasmic genomes of wheat and Setaria italica. Genome. 2004. 47:680-688
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48.Fengning Xiang, Guangmin Xia, Humin Chen. Asymmetric Somatic Hybridization Between wheat (Triticum aestivum) and Avena sativa L. Science in China. 2003. 46 (3): 243-252
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国家发明专利
1. 大豆萌动胚真空渗透辅助的外源基因转化方法，2010，ZL201010223264.5
2. 一种农杆菌介导的甘薯不定芽转化方法，2009，ZL200910017332.X
3. 芦苇植物络合素基因PaPCS及其应用，2011，201110189821.0
4.大豆圣豆9号NAC转录因子基因GmST1及其应用，2013，ZL201210128273.5
5. 大豆圣豆9号NAC转录因子基因GmST2及其应用，2013，ZL201210128590.7
6. 一种对金银花不定芽转化获得转基因植株的方法，2014，ZL201310284657.0
7. 大豆圣豆9号GmNAC4基因盐诱导启动子，2016，ZL201410621142X
9. 金银花HQT基因冷诱导启动子，2017，ZL201510613599.0
10. 大豆威廉姆斯82 中NAC膜结合转录因子基因GmNTL1及其应用，2018，ZL201610867847.9
11. 一种大豆圣豆9号GmNAC15基因的ABA诱导启动子，2018，ZL201611091573.5
12. 大豆威廉姆斯82 中NAC膜结合转录因子基因GmNTL7及其应用，2018，ZL201610867930.6
13. 一种大豆高效启动子盐诱导功能元件及其应用，2019，ZL201710112865.0
14. 大豆盐诱导型人工合成启动子AP2及其应用，2021，ZL201811061218.2
15. 一种大豆菏豆12GmRBOH1基因盐诱导启动子及其应用，2022，ZL201911235410.3
16. 一种大豆圣豆9号GmPK6基因盐诱导启动子及其应用，2022，ZL201910170916.4
17. 大豆WD40蛋白基因GmPRL1b及其应用，2022，ZL202011078669.4
18. 与干旱胁迫相关的菏豆 12 号GmYLD1基因及其等位突变基因与应用，2022， ZL2019112368021
19. 与籽粒及株高发育相关的大豆GmDSB1基因及其突变体与应用，2023，ZL 20221 1009700.8
20. 与大豆花发育相关的菏豆12 号GmDFB1 基因及其突变体与应用，2023， ZL202211009736.6
